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Image Search Results
Journal: PLoS ONE
Article Title: Ambient Oxygen Promotes Tumorigenesis
doi: 10.1371/journal.pone.0019785
Figure Lengend Snippet: A. Decreased oxidative DNA damage as measured by 8-oxoG (pg/µg genomic DNA) ELISA in polyp-free segments of intestine from 10% compared to 21% oxygen APC Min/+ mice. Data shown as mean ± SEM, with n = 3. B. Decreased DNA damage response observed by γ-H2AX western blotting in polyp-free intestine of 10% compared to 21% oxygen APC Min/+ mice. Relative intensity quantified by densitometric scanning of γ-H2AX normalized to β-actin protein (graph), n = 3. C. Decreased protein levels of β-catenin and its transcriptional target cyclin D1 in polyp-free intestine of 10% compared to 21% oxygen APC Min/+ mice. Representative samples in each oxygen condition and β-actin serves as protein loading control, n = 3.
Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Control
Journal: PLoS ONE
Article Title: Ambient Oxygen Promotes Tumorigenesis
doi: 10.1371/journal.pone.0019785
Figure Lengend Snippet: A. Decreased oxidative DNA damage as measured by 8-oxoG (pg/µg genomic DNA) ELISA in polyp-free segments of intestine from 10% compared to 21% oxygen APC Min/+ mice. Data shown as mean ± SEM, with n = 3. B. Decreased DNA damage response observed by γ-H2AX western blotting in polyp-free intestine of 10% compared to 21% oxygen APC Min/+ mice. Relative intensity quantified by densitometric scanning of γ-H2AX normalized to β-actin protein (graph), n = 3. C. Decreased protein levels of β-catenin and its transcriptional target cyclin D1 in polyp-free intestine of 10% compared to 21% oxygen APC Min/+ mice. Representative samples in each oxygen condition and β-actin serves as protein loading control, n = 3.
Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma);
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Control